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scanning confocal laser microscopy (sclm  (Bio-Rad)


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    Bio-Rad scanning confocal laser microscopy (sclm
    (A) Photograph of a piece of old, dried, consolidated hydrozincite with dark spots, with cyanobacterium Scytonema sp. in a dormant state. Bar, 0.5 cm. (B) The same piece of hydrozincite after a period of incubation in BG-11 medium (diluted 1:10) under dim light, clearly showing growth of Scytonema sp., which was entrapped in the hydrozincite matrix and which forms blue-green filaments. Bar, 0.5 cm. (C) In the transmission mode the Scytonema sp. clearly grows out of the hydrozincite tubing. A thick-wall heterocyst (short arrow) is clearly visible. Bar, 12, μm. (D) The same image by <t>SCLM</t> shows single autofluorescent cells of a Scytonema sp. Autofluorescence is due to chlorophyll a encapsulated in the sheath. Where the heterocyst occurs (short arrow), no autofluorescence appears, since the photosynthetic apparatus is degenerated. Moreover, hydrozincite <t>emits</t> <t>fluorescence</t> (long arrow). Bar, 12 μm. (F) Coculture of Scytonema sp. and the microalga Chlorella sp. The cyanobacterium shows filaments of different diameters. Bar, 12 μm. (G) The same image taken by SCLM (the sheath of the Scytonema sp. becomes empty and wide with aging and autofluorescence [arrow]). Bar, 12 μm.
    Scanning Confocal Laser Microscopy (Sclm, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scanning confocal laser microscopy (sclm/product/Bio-Rad
    Average 90 stars, based on 1 article reviews
    scanning confocal laser microscopy (sclm - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Heavy Metal Coprecipitation with Hydrozincite [Zn 5 (CO 3 ) 2 (OH) 6 ] from Mine Waters Caused by Photosynthetic Microorganisms"

    Article Title: Heavy Metal Coprecipitation with Hydrozincite [Zn 5 (CO 3 ) 2 (OH) 6 ] from Mine Waters Caused by Photosynthetic Microorganisms

    Journal:

    doi:

    (A) Photograph of a piece of old, dried, consolidated hydrozincite with dark spots, with cyanobacterium Scytonema sp. in a dormant state. Bar, 0.5 cm. (B) The same piece of hydrozincite after a period of incubation in BG-11 medium (diluted 1:10) under dim light, clearly showing growth of Scytonema sp., which was entrapped in the hydrozincite matrix and which forms blue-green filaments. Bar, 0.5 cm. (C) In the transmission mode the Scytonema sp. clearly grows out of the hydrozincite tubing. A thick-wall heterocyst (short arrow) is clearly visible. Bar, 12, μm. (D) The same image by SCLM shows single autofluorescent cells of a Scytonema sp. Autofluorescence is due to chlorophyll a encapsulated in the sheath. Where the heterocyst occurs (short arrow), no autofluorescence appears, since the photosynthetic apparatus is degenerated. Moreover, hydrozincite emits fluorescence (long arrow). Bar, 12 μm. (F) Coculture of Scytonema sp. and the microalga Chlorella sp. The cyanobacterium shows filaments of different diameters. Bar, 12 μm. (G) The same image taken by SCLM (the sheath of the Scytonema sp. becomes empty and wide with aging and autofluorescence [arrow]). Bar, 12 μm.
    Figure Legend Snippet: (A) Photograph of a piece of old, dried, consolidated hydrozincite with dark spots, with cyanobacterium Scytonema sp. in a dormant state. Bar, 0.5 cm. (B) The same piece of hydrozincite after a period of incubation in BG-11 medium (diluted 1:10) under dim light, clearly showing growth of Scytonema sp., which was entrapped in the hydrozincite matrix and which forms blue-green filaments. Bar, 0.5 cm. (C) In the transmission mode the Scytonema sp. clearly grows out of the hydrozincite tubing. A thick-wall heterocyst (short arrow) is clearly visible. Bar, 12, μm. (D) The same image by SCLM shows single autofluorescent cells of a Scytonema sp. Autofluorescence is due to chlorophyll a encapsulated in the sheath. Where the heterocyst occurs (short arrow), no autofluorescence appears, since the photosynthetic apparatus is degenerated. Moreover, hydrozincite emits fluorescence (long arrow). Bar, 12 μm. (F) Coculture of Scytonema sp. and the microalga Chlorella sp. The cyanobacterium shows filaments of different diameters. Bar, 12 μm. (G) The same image taken by SCLM (the sheath of the Scytonema sp. becomes empty and wide with aging and autofluorescence [arrow]). Bar, 12 μm.

    Techniques Used: Incubation, Transmission Assay, Fluorescence



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    scanning confocal laser microscopy (sclm  (Bio-Rad)
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    Bio-Rad scanning confocal laser microscopy (sclm
    (A) Photograph of a piece of old, dried, consolidated hydrozincite with dark spots, with cyanobacterium Scytonema sp. in a dormant state. Bar, 0.5 cm. (B) The same piece of hydrozincite after a period of incubation in BG-11 medium (diluted 1:10) under dim light, clearly showing growth of Scytonema sp., which was entrapped in the hydrozincite matrix and which forms blue-green filaments. Bar, 0.5 cm. (C) In the transmission mode the Scytonema sp. clearly grows out of the hydrozincite tubing. A thick-wall heterocyst (short arrow) is clearly visible. Bar, 12, μm. (D) The same image by <t>SCLM</t> shows single autofluorescent cells of a Scytonema sp. Autofluorescence is due to chlorophyll a encapsulated in the sheath. Where the heterocyst occurs (short arrow), no autofluorescence appears, since the photosynthetic apparatus is degenerated. Moreover, hydrozincite <t>emits</t> <t>fluorescence</t> (long arrow). Bar, 12 μm. (F) Coculture of Scytonema sp. and the microalga Chlorella sp. The cyanobacterium shows filaments of different diameters. Bar, 12 μm. (G) The same image taken by SCLM (the sheath of the Scytonema sp. becomes empty and wide with aging and autofluorescence [arrow]). Bar, 12 μm.
    Scanning Confocal Laser Microscopy (Sclm, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scanning confocal laser microscopy (sclm/product/Bio-Rad
    Average 90 stars, based on 1 article reviews
    scanning confocal laser microscopy (sclm - by Bioz Stars, 2026-03
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    Image Search Results


    (A) Photograph of a piece of old, dried, consolidated hydrozincite with dark spots, with cyanobacterium Scytonema sp. in a dormant state. Bar, 0.5 cm. (B) The same piece of hydrozincite after a period of incubation in BG-11 medium (diluted 1:10) under dim light, clearly showing growth of Scytonema sp., which was entrapped in the hydrozincite matrix and which forms blue-green filaments. Bar, 0.5 cm. (C) In the transmission mode the Scytonema sp. clearly grows out of the hydrozincite tubing. A thick-wall heterocyst (short arrow) is clearly visible. Bar, 12, μm. (D) The same image by SCLM shows single autofluorescent cells of a Scytonema sp. Autofluorescence is due to chlorophyll a encapsulated in the sheath. Where the heterocyst occurs (short arrow), no autofluorescence appears, since the photosynthetic apparatus is degenerated. Moreover, hydrozincite emits fluorescence (long arrow). Bar, 12 μm. (F) Coculture of Scytonema sp. and the microalga Chlorella sp. The cyanobacterium shows filaments of different diameters. Bar, 12 μm. (G) The same image taken by SCLM (the sheath of the Scytonema sp. becomes empty and wide with aging and autofluorescence [arrow]). Bar, 12 μm.

    Journal:

    Article Title: Heavy Metal Coprecipitation with Hydrozincite [Zn 5 (CO 3 ) 2 (OH) 6 ] from Mine Waters Caused by Photosynthetic Microorganisms

    doi:

    Figure Lengend Snippet: (A) Photograph of a piece of old, dried, consolidated hydrozincite with dark spots, with cyanobacterium Scytonema sp. in a dormant state. Bar, 0.5 cm. (B) The same piece of hydrozincite after a period of incubation in BG-11 medium (diluted 1:10) under dim light, clearly showing growth of Scytonema sp., which was entrapped in the hydrozincite matrix and which forms blue-green filaments. Bar, 0.5 cm. (C) In the transmission mode the Scytonema sp. clearly grows out of the hydrozincite tubing. A thick-wall heterocyst (short arrow) is clearly visible. Bar, 12, μm. (D) The same image by SCLM shows single autofluorescent cells of a Scytonema sp. Autofluorescence is due to chlorophyll a encapsulated in the sheath. Where the heterocyst occurs (short arrow), no autofluorescence appears, since the photosynthetic apparatus is degenerated. Moreover, hydrozincite emits fluorescence (long arrow). Bar, 12 μm. (F) Coculture of Scytonema sp. and the microalga Chlorella sp. The cyanobacterium shows filaments of different diameters. Bar, 12 μm. (G) The same image taken by SCLM (the sheath of the Scytonema sp. becomes empty and wide with aging and autofluorescence [arrow]). Bar, 12 μm.

    Article Snippet: The white solid specimen was soaked in sterile buffered water and then observed without any treatment under light transmission and in fluorescence modes with scanning confocal laser microscopy (SCLM) (Bio-Rad Microscience Division; model MRC-500); the microscope was equipped with a krypton-argon laser, with maximum emission at 488 nm.

    Techniques: Incubation, Transmission Assay, Fluorescence